Figure 2.

Calibration protocol to create a cell-specific ArchT model. (A) The calibration protocol consists of changing the light intensity and the holding potential to determine the ArchT model parameters for an individual cell, specifically the light dependence using the light-intensity ramp portion of the protocol (illustrated in blue) and voltage dependence using the voltage-clamp steps in the protocol (illustrated in purple). The bottom panel illustrates an example of the current output measured via patch clamp for one cell during the calibration protocol. (B and C) Currents from the example trace in (A) during the light-intensity ramp and voltage-clamp steps, respectively, are shown. The currents were subtracted from the baseline, defined as an average of 10 ms before illumination.