Fig. 3.

NZX characteristics. (A) Concentration related MTT cytotoxicity analysis of NZX in M. tuberculosis H37Rv-infected primary macrophages measured 6 days post treatment. Cytotoxicity assays of NZX (100 μM) treated primary macrophages as determined by (B) ATPlite and (C) Prestoblue. (D) Inflammatory response was measure by NF-κB-activation in monocytes after addition of NZX (12.5 μM) or 10 ng/ml LPS (mean +sd, N = 3, ***p < 0.001, ns = not significant.). (E) Enzymatic degradation of NZX by the human proteases Cathepsin G, alpha-Thrombin and Human Neutrophil Elastase (HNE) after six hours of incubation. Results are depicted as mean ± 95% CI of three independent experiments.