Fig. 16. PNs for delivery of active proteins into cells. (a) Delivery of tumor suppressor p53 to induce apoptosis via Caspase 3/7 activation. Adapted with permission from ref. 51. 2014 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. (b) Intracellular delivery of β-galactosidase into A549 cancer cells. The blue coloration of the cells showed that the enzyme preserves its bioactivity in vitro. Adapted with permission from ref. 20. Copyright 2013 American Chemical Society. (c) Selective delivery of C3 into cell lines overexpressing somatostatin receptors was shown compared to a receptor negative cell line. The application of the fusion protein in chick embryo chorioallantoic membrane model showed an antiangiogenic effect. Adapted with permission from ref. 62. Copyright 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. (d) The internalization of a fluorescently labelled LiDPS protein cage into the microbial pathogen, Staphylococcus aureus, was mediated by assembly with an antibody. Adapted with permission from ref. 19. Copyright 2009 American Chemical Society. (e) CD3xHer2 bispecific antibody target Her2-positive human breast cancer SKBR3 cells increase cytotoxicity due to T-cell mediated lysis. Adapted with permission from ref. 199. Copyright 2014 American Chemical Society.