Fig. 4. Genetically modified PBs. (a) Cytochrome b562 63Cys mutant with unpaired thiol. (b) Cytochrome cb562 variants with i/i + 4 His (left) or quinolate (right) mutations. Adapted with permission from ref. 79 and 80. Copyright 2007, 2010 American Chemical Society. (c) Glutathione-S-transferase with mutations in antiparallel or “V” orientation. Adapted with permission from ref. 29 and 84. Copyright 2017, 2012 Royal Society of Chemistry. (d) Alkaline phosphatase with mutations incorporating reactive peptide tags along the short or long axis of the protein. Adapted with permission from ref. 85 and 86. Copyright 2011 Royal Society of Chemistry. Protein images (PDB: ; 1EW9) were adapted from the NGL viewer.240.
