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. Author manuscript; available in PMC: 2018 Dec 11.
Published in final edited form as: ACS Infect Dis. 2016 Sep 27;2(11):827–838. doi: 10.1021/acsinfecdis.6b00124

Figure 2.

Figure 2.

P. berghei infection depletes BH4 and lowers the BH4/BH2 ratio in plasma, red blood cells, and brain tissue. Plasma, erythrocyte, and tissues BH4 and 7,8-BH2 were determined by HPLC on day 6 postinoculation. P. berghei ANKA-infected mice received a continuous intravenous infusion of saline (n = 9) or 2.4 g kg−1 day−1 citrulline + 15 mg kg−1 day−1 sepiapterin (n = 11) from day −1 to day 6 postinoculation. Tissue concentrations were normalized to soluble protein. Saline-infused uninfected mice (n = 12) served as controls. Data are pooled from three independent experiments. Box plots depict the median and IQR. Whiskers extend to the highest and lowest values within 1.5IQR of the 75th and 25th percentiles, and values outside this range were plotted as individual points (Tukey’s method). *p < 0.05, **p < 0.01, and ***p < 0.001 by the Mann–Whitney test. ns (not significant): p > 0.05.