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. Author manuscript; available in PMC: 2019 Mar 19.
Published in final edited form as: Angew Chem Int Ed Engl. 2018 Feb 22;57(13):3391–3395. doi: 10.1002/anie.201713167

Figure 1. Peptide-based nanodiscs as a tool to unveil lipid-boundary regions of microsomal CYP2B4.

Figure 1.

(a) Schematic of the lipid-exchange experiment. CYP2B4 was reconstituted with a 2.5-fold excess of empty nanodiscs and incubated overnight to allow lipid exchange. (b) SEC profiles showing two partially overlapping peaks, representing the reconstituted and empty fractions, respectively; the protein-containing fraction was also characterized by the absorbance at the Soret maximum (417 nm).