Figure 5. Polycomb activity regulates early transcriptional response after nerve injury.

A, RNA-seq analysis identified a number of genes dysregulated by Eed cKO in uninjured or 1 d after injury conditions. Gene expression was determined by RNA-seq analysis of 3 samples per genotype and condition (p-value, < 0.05). The brackets indicate the number of genes associated with H3K27me3 around the transcription start site (±7 Kb). B, C, RNA-seq analysis was used to identify the expression level of early and late injury genes in Eed cKO nerves and control nerves at 1 d post-cut relative to uninjured control nerves. Note that the y-axis is on a log scale. Data: mean of n=3 with p-value, < 0.05 per genotype. The late injury genes were identified from microarray analysis of peripheral nerves 3, 5 or 7 d after injury (> 2 fold) (GEO accession: GSE22291, GSE38693, GSE33454) (Barrette et al., 2010; Arthur-Farraj et al., 2012; Kim et al., 2012)(Kim et al. 2012). See Supporting Information Table 3 and 4 for the complete list of genes analyzed in Figure 5.