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. Author manuscript; available in PMC: 2018 Dec 11.
Published in final edited form as: Appl Microbiol Biotechnol. 2018 May 23;102(15):6547–6565. doi: 10.1007/s00253-018-9062-x

Figure 1:

Figure 1:

Schematic showing the recombinant proteins that were expressed in B. subtilis. Each protein contains a signal peptide from B. subtilis PhrC (SP), a hexahistidine tag (His6), the Clostridium thermocellum endoglucanase Cel8A catalytic domain (Cel8A), and the LysM cell wall binding domain from B. subtilis LytE protein (LysM). In some proteins the enzyme and cell wall binding domains are joined by a polypeptide linker derived from the Staphylococcus aureus FnBPB (linker). GenBank accession numbers are given in the materials and methods.