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. 2018 May 31;39(10):1645–1660. doi: 10.1038/aps.2017.192

Figure 5.

Figure 5

Downregulation of p62 enhanced escin-induced cell apoptosis. (A–B) The effect of escin on cell viability after p62 knockdown. Cells were transiently transfected with scramble siRNA or p62 siRNA1/2 for 36 h and then treated with 60 μg/mL escin for 12 h. Cell viability was assessed by CCK8 assay. (C–D) Analysis of apoptosis with FACS in HCT116 cells. Cells were treated as described above, and apoptosis was analyzed with FACS. Quantitative analysis of apoptosis after p62 knockdown with or without treatment of escin is shown (D). (E–H) Expression of apoptosis related proteins. Cells were treated as described above, and apoptosis-related proteins were detected with Western blotting (E and F). Quantitative analysis of the optical density ratio of cleaved-PARP and cleaved-caspase 9 compared with β-actin is shown (G and H). ns P>0.05, * P<0.05, ** P<0.01 vs the corresponding group.