Fig. 1.

Construction and characterization of the recombinant EGFR-targeting β-defensin Ec-LDP-hBD1. a Diagram of the NdeI/XhoI gene fragments encoding for the protein Ec-LDP-hBD1 (top) and the amino acid sequence of Ec-LDP-hBD1 (bottom). b Expression analysis of the protein Ec-LDP-hBD1 by 12% SDS-PAGE. Lane 1, molecular weight marker; Lane 2, the first step of purified protein Ec-LDP-hBD1 by His Trap affinity columns; Lane 3, the second step of purified protein Ec-LDP-hBD1 by His Trap affinity columns; Lane 4, the third step of purified protein Ec-LDP-hBD1 by His Trap affinity columns; Lane 5, the fourth step of purified protein Ec-LDP-hBD1 by His Trap affinity columns. c Western blot detection of the fusion protein Ec-LDP-hBD1 using mouse anti-His-tag monoclonal antibody (1/1000 dilution) and HRP-conjugated goat anti-mouse IgG (1/2000 dilution). Lanes 1 and 2, western blot detection of the fusion proteins Ec-LDP-hBD1. d HPLC analysis of protein Ec-LDP-hBD1 purity