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. 2018 May 10;39(9):1513–1521. doi: 10.1038/aps.2017.184

Figure 7.

Figure 7

AT1R Knockdown blocks SND p102-induced AT1R/ERK/Smad3 signaling in cultured MCs. (A) Cells were transfected with pcDNA3.0-SND p102 plasmid (1 μg) or pcDNA3.0 plasmid (1 μg) for 48 h. p-ERK and p-Smad3 protein levels were measured by Western blot analysis. (B) A 50 nmol/L aliquot of AT1R siRNA or control siRNA and 1 μg of pcDNA3.0-SND p102 plasmid or pcDNA3.0 plasmid were cotransfected into cells for 48 h. AT1R, p-ERK, and p-Smad3 levels were measured by Western blot analysis. Data represent the mean±SEM of at least 3 experiments. * P<0.05, ** P<0.01 compared with pcDNA3.0 plasmid- or pcDNA3.0 plasmid and control siRNA-transfected samples. ## P<0.01 compared with pcDNA3.0-SND p102 plasmid- and control siRNA-transfected samples.