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The immunoregulatory properties of hAECs and functional identification of the CD90+ population. Whole hAECs or sorted CD90+ and CD90- hAECs were co-cultured with PHA-activated PBMCs for 3 d. TNFα, IL2, IL17 and IL10 in the culture medium were examined by ELISA. (A) Representative flow cytometry gating for CD90+ and CD90- hAEC sorting. (B–E) ELISA detection of the cytokines TNFα, IL10, IL17 and IL2 in the medium of hAECs.
