Fig. 2.

Integrity and activity of mTORC2 were elevated in HUVEC replicative senescence. a The young HUVECs (passage 3) and replicative senescent HUVECs (passage 11) were subjected to β-gal staining and b immunoblotting to determine the p21 protein level, n = 5. c Western blotting analysis for the detection of expression of mTOR, Rictor, and p-mTOR-S2481, n = 3. d Co-immunoprecipitation was performed to detect the interaction between mTOR and Rictor, n = 3. e Detection of expression of p-Akt-S473 and Akt by Western blotting, n = 5. β-Actin served as the loading control. The bar graphs show the expression levels. Data are shown as the means ± S.E.M. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control group