Figure 2.

Emodin ameliorated asthmatic lung injury in DRA-induced asthma. Lung lobes were excised, fixed and processed for histological evaluation. (A) H&E staining was performed to determine the asthmatic inflammation in DRA-induced asthmatic inflammation in response to vehicle or emodin treatment. The black arrows indicate pulmonary infiltrated eosinophils. (B) PAS staining was performed for the identification of goblet cells in the epithelium. Black arrows indicate PAS-positive goblet cells. (C) The number of PAS-positive cells per 100 pixel lengths of the bronchiole was determined as described in the materials and methods section. The results represent mean±SEM (n=5 mice for each group), and ND stands for 'not detected'. ^* P<0.05 between the indicated groups.