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. 2018 Feb 8;39(8):1317–1325. doi: 10.1038/aps.2017.147

Figure 6.

Figure 6

Emodin reduced the phosphorylation of STAT6 and the expression of KLF4 in IL-4-induced BMDMs. BMDMs isolated from WT mice were pretreated with emodin at 0, 2, 10 and 50 μmol/L for 30 min and then stimulated with 10 ng/mL of IL-4. Next, Western blotting was performed to detect the expression of STAT6 and KLF4. (A) The phosphorylation of STAT6 and total STAT6 was detected at 30 min after IL-4 stimulation. (C) The expression of KLF4 and GAPDH was detected by Western blotting. The ratio of phosphorylated and total STAT6 (B) and the ratio of KLF4 and GAPDH (D) in densitometry were analyzed using ImageJ software. Statistical analysis was performed using Student's t test. n=3. * P<0.05, ** P<0.01 between the indicated groups.