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. 2018 Apr 19;39(9):1439–1452. doi: 10.1038/aps.2017.203

Figure 1.

Figure 1

Cyanidin attenuates Aβ-induced NF-κB pathway activation and NO production in SK-N-SH cells. The SK-N-SH cells were pretreated with 20 μmol/L of cyanidin or 20 μmol/L of NAC for 2 h prior to treatment with 10 μmol/L Aβ25-35 for 24 h. The expression levels of proteins related to the NF-κB pathway were analyzed by Western blot. Western blotting analysis was performed to measure the total, cytosolic, and nuclear protein levels of p65 (A); IκBα (B) and iNOS (C); cytosolic proteins were normalized to actin, the nuclear proteins were normalized to lamin B1. The Griess reaction assay was performed to determine the level of nitrite production (D). The data are expressed as the mean±SEM (n=3). ** P<0.01 vs the control. ## P<0.01 vs the Aβ25-35-treated group.