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. 2018 Apr 19;39(9):1439–1452. doi: 10.1038/aps.2017.203

Figure 2.

Figure 2

Cyanidin attenuates Aβ-induced reduction of Nrf2 pathway activation and SOD activity in SK-N-SH cells. The SK-N-SH cells were pretreated with 20 μmol/L of cyanidin or 20 μmol/L of NAC for 2 h prior to treatment with 10 μmol/L Aβ25-35 for 24 h. The expression levels of proteins related to the Nrf2 pathway were analyzed by Western blot. Western blotting analysis was performed to measure the total, cytosolic, and nuclear protein levels of Nrf2 (A); the expression of the antioxidant enzyme genes including HO-1, NQO-1, and GCLC (B); actin to normalize the cytosolic proteins; and lamin B1 to normalize the nuclear proteins. A SOD activity assay was carried out to determine the level of SOD activity (C). The SK-N-SH cells were pretreated with 20 μmol/L OTA for 1 h; preincubation was carried out with cyanidin (20 μmol/L) or NAC (20 μmol/L) for 2 h, and the cells were exposed to Aβ25-35 (10 μmol/L) for another 24 h. The Griess reaction assay was performed to determine the level of nitrite production (D). The data are expressed as the mean±SEM (n=3). ** P<0.01 vs the control. ## P<0.01 vs the Aβ25-35-treated group.