Figure 3A-D.

Cyanidin protects against Aβ-induced PI3K/Akt pathway activation but the effect has no relation to inflammation in SK-N-SH cells. SK-N-SH cells were pretreated with or without 25 μmol/L LY294002 for 1 h. Then, the cells were pretreated with 20 μmol/L cyanidin or 20 μmol/L NAC for 2 h prior to treatment with 10 μmol/L Aβ_25-35 for 24 h. Western blotting analysis was performed to measure the protein expression levels of p-Akt and Akt, and protein loading was normalized to Akt (A). The SK-N-SH cells were pretreated with 25 μmol/L LY294002 for 1 h; preincubation was carried out with cyanidin (20 μmol/L) or NAC (20 μmol/L) for 2 h, and the cells were exposed to Aβ_25-35 (10 μmol/L) for another 24 h. Western blotting analysis was performed to measure the total, cytosolic, and nuclear protein levels of p65 (B), IκBα (C), iNOS (D). The data are expressed as the mean±SEM (n=3). ^** P<0.01 vs the control. ^# P<0.05, ^## P<0.01 vs the Aβ_25-35-treated group. ^$$ P<0.01 vs the Aβ_25-35-plus-inhibitor-treated group.