Skip to main content
. 2018 Feb 8;39(8):1326–1337. doi: 10.1038/aps.2017.189

Figure 3.

Figure 3

Heparanase increases the expression of adhesion molecules. (A) MDA-MB-231 cells stably transfected with mock or heparanase-overexpression plasmid were subjected to immunoblotting with indicated antibodies. (B) MDA-MB-231 cells depleted of heparanase using CRISPR/Cas9 technology were subjected to immunoblotting with the indicated antibodies. (C) Adhesion molecules localization. MDA-MB-231 cell clusters were stained for nuclei (blue), FAK (red), paxillin (green) or ICAM-1 (white) by immunofluorescence assay. Bar, 10 μm. (D) MDA-MB-231 cells transfected with FAK-specific siRNAs or treated for 12 h with PF-562271 at the indicated concentrations were subjected to immunoblotting with indicated antibodies. (E) MDA-MB-231 cells transfected with ICAM-1-specific siRNAs or treated for 12 h with A-205804 at the indicated concentrations were subjected to immunoblotting with indicated antibodies. Densitometric data (fold) were shown below each group.