Figure 6.

JG6 inhibits breast cancer metastasis. (A) MDA-MB-231 cells were treated with various concentrations of JG6 for 48 h and injected into the tail vein of mice. Shown were representative images of tumor cells in the blood captured by fluorescence and DIC microscopy. Bar graph showed the mean percentage of single cells or cell clusters in the groups, left untreated was served as control. (B) Representative images of H&E-stained sections of mouse lungs. Histogram showing the inhibitory effect of JG6 on the number of lung metastatic foci (n=6). (C) Proposed working model. Heparanase activates FAK-Src-paxillin and ICAM-1 signaling and promotes cell cluster formation, which increases tumor metastasis. JG6 inhibits this process induced by heparanase. Values are expressed as the mean±SD, One-way ANOVA was applied in experiments containing multiple groups in (A) and (B).