Roles of efflux transporters in the interaction between apatinib and docetaxel. (A) Effect of apatinib on the accumulation of digoxin, SN-38 and CDCF in Caco-2 cells. (B) Effect of LY335979, MK571 and Ko143 on the accumulation of docetaxel in Caco-2 cells. (C) Effect of apatinib and LY335979 on the accumulation of docetaxel in A549/DTX and A549 cells. (D) Effect of apatinib and Ko143 on the accumulation of docetaxel in A549/DTX and A549 cells. LY335979 (5 μmol/L), MK571 (5 μmol/L) and Ko143 (5 μmol/L) are specific inhibitors of P-gp, MRP2 and BCRP, respectively. Digoxin, SN-38 and CDCF are specific substrates of P-gp, BCRP and MRP2, respectively. *
P<0.05, **
P<0.01 vs vehicle.