GIRK1/2-dependent DMR
response to ML297 can be diminished using
known GIRK channel inhibitors. (a,c,e) Exemplary DMR traces of ML297-stimulated
HEK cells stably expressing GIRK1/2 channels after preincubation with
indicated concentrations of ionic barium, SCH, or TPNQ. (b,d,f) Concentration-effect
relationships calculated from AUC of DMR recordings (n = 3) (a,c,e), between 0–4800 (a), 300–3900 (c), and
0–8000 s (e). For pEC50 values, see Table S2. (g–i) AUC quantification of DMR traces of PGE1 (100
μM) and ATP (100 μM) (n = 2), calculated
between 0–4800 (g), 300–3900 (h), and 0–8000
s (i).