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. 2018 Dec 11;13(12):e0208550. doi: 10.1371/journal.pone.0208550

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© 2018 Mills et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — Mammary glands (5–7 mice per timepoint, 2 mammary glands per mouse) were harvested in diestrus from 5, 8 and 12 week old animals. (A) Carmine alum stained whole mounts of mammary glands from wildtype and Gas6^-/- animals. Scale bar = 1 mm. (B) Ductal elongation was quantified by measuring the distance from lymph node to the most distal tip of a duct or TEB. (5 wk p = 0.08; 8 wk p = 0.06; 12 wk p = 0.86). (C) Quantification of TEB number in wildtype and Gas6^-/- glands (p = 0.49). (D) Branching morphogenesis was quantified by counting the branch points in the entire gland (5 wk p = 0.48; 8 wk p = 0.48; 12 wk p = 0.11).