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. 2018 Dec 11;13(12):e0208769. doi: 10.1371/journal.pone.0208769

Table 3. Laboratory items sterilized in this study, the growth conditions used to assess sterility, and the time required to sterilize each item in all pressure cookers tested.

Item to be sterilized Media used to assess sterilization Growth temperature Minimum sterilization time (mins)
Nutrient broth - 30°C 15
Nutrient agar - 30°C 15
Soil water NA and SDA 30°C 45
15-cm Mall probe and seekers NA and SDA 30°C 15
15-cm reagent digger spatulas NA and SDA 30°C 15
Dissection scissors NA and SDA 30°C 30

Abbreviations: NA, nutrient agar; SDA, Sabouraud dextrose agar