Table 3. Laboratory items sterilized in this study, the growth conditions used to assess sterility, and the time required to sterilize each item in all pressure cookers tested.
Item to be sterilized | Media used to assess sterilization | Growth temperature | Minimum sterilization time (mins) |
---|---|---|---|
Nutrient broth | - | 30°C | 15 |
Nutrient agar | - | 30°C | 15 |
Soil water | NA and SDA | 30°C | 45 |
15-cm Mall probe and seekers | NA and SDA | 30°C | 15 |
15-cm reagent digger spatulas | NA and SDA | 30°C | 15 |
Dissection scissors | NA and SDA | 30°C | 30 |
Abbreviations: NA, nutrient agar; SDA, Sabouraud dextrose agar