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. 2018 Nov 29;14(11):e1007816. doi: 10.1371/journal.pgen.1007816

Fig 1. BLM is important for suppressing Flex1-induced mitotic recombination.

Fig 1

In all experiments, error bars represent standard deviation (SD) of at least three independent experiments. The P value is indicated as *P<0.05 and **P<0.01, and n.s. is not significant. A. Schematic drawing of the EGFP-based HR-Flex and HR-Luc reporters as previously described [21]. Luc: luciferase fragment; D-EGFP: donor EGFP. B. U2OS cells containing the HR-Flex reporter (left) or HR-Luc reporter (right) were infected by lentiviruses encoding BLM shRNAs or a control vector (Ctrl), and spontaneous mitotic recombination was assayed on indicated days. The expression of BLM was examined by Western blot with β-actin as loading control. C. U2OS (HR-Flex) cells expressing BLM shRNA or vector (Ctrl) were generated by lentiviral infection followed by drug selection and two days later, cells were treated with 2 mM HU for 24 hr and assayed for EGFP-positive events 72 hr later. D. Spontaneous recombination was examined in U2OS (HR-16C/AT1) and U2OS (HR-16C/AT3) cells after culturing pre-sorted non-green cells for indicated days. 16C/AT1 and 16C/AT3 are AT rich sequences derived from FRA16C. The expression of BLM is shown by Western blot with β-actin as loading control.