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. 2018 Nov 29;14(11):e1007832. doi: 10.1371/journal.pgen.1007832

Fig 2. Sporulation analysis of various ndt80 mutants in dmc1Δ and DMC1 diploids.

Fig 2

Potential Mek1 phosphorylation sites are indicated in green, purple and gold, corresponding to the protein domain and have the sequence RXXT/S. Asterisks indicate non-consensus sites detected as phosphorylated in global phosphoproteomic analyses of dmc1Δ- arrested cells [73]. Sporulation was assayed after three days at 30ºC on solid sporulation medium. Each row represents a diploid homozygous for a different allele of NDT80 with mutated residues indicated by either A (alanine), D (aspartic acid) or N (asparagine). “nd” indicates no data. (NDT80, pHL8; NDT80-Δbc, pHL8-Δbc; ndt80-ΔRPSKR, pNH317; ndt80-KR>AA, pHL8-KR>AA; ndt80-KR>DD, pHL8-KR>DD; ndt80-2A, pHL8-2A; ndt80-4AMS, pHL8-4AMS; ndt80-5AMS; pHL8-5AMS; ndt80-7AMS, pHL8-7AMS; ndt80-9AMS, pHL8-9AMS; ndt80-10AMS, pHL8-10AMS; ndt80-8A, pNH405; ndt80-6A, pNH400; ndt80-10DMS, pHL8-10DMS; ndt80-S24D, pHL8-S24D; ndt80-S343D, pHL8-S343D; ndt80-S205D T211D, pHL8-S205D T211D; ndt80-S327D S329D, pHL8-S327D S329D; ndt80-6D, pNH401; ndt80-6N, pHL8-6N. Sporulation was scored in either a dmc1Δ (NH2402) or DMC1 diploid (NH2081). Values in magenta are significantly higher than dmc1Δ NDT80 with p values <9 X10-10, while values in green are significantly lower than DMC1 NDT80 with p values < 0.014 (ndt80-6A) or 9.58 X 10−8. p values were determined using a one-sided Fisher’s Exact Test. Strains, standard deviations, number of biological replicates and p values are listed in Data S1.