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. 2018 Apr 16;5(2):e91. doi: 10.14440/jbm.2018.243

Figure 1. Tissue digestion, cell isolation and cell pellet preparation.

Figure 1.

A. Surgically removed UL. B. UL is minced in a 10 cm petri dish. C. UL tissue pieces are digested in a baffled flask that is set in a shaker-incubator. D. UL cells are plated in 10 cm culture dishes. E. Cell pellets are suspended in DMEM/F12.