Table 2. Sub-strains generated from DS10 monomer isolated by SEC or cutoff filter.
Tau RD-YFP monomer (Ms) was isolated from DS10 by either by SEC or 100kD cutoff filter and inoculated into DS1 to create sub-strains. Multiple clones were isolated and characterized by morphology. Columns indicate the number of clones identified (n) and the percentage this represents of the total (%). Isolation of Ms from DS10 by SEC or 100 kD cutoff filter each enabled a similar proportion of sub-strains to form. Classification of cell morphology was performed using blinded analysis.
| Ms | SEC | 100kD filter | ||
|---|---|---|---|---|
| N | % | N | % | |
| 10.1 | 19 | 36 | 12 | 43 |
| 10.2 | 11 | 21 | 3 | 11 |
| 10.3 | 7 | 13 | 5 | 20 |
| 10.4 | 5 | 9 | 3 | 11 |
| 10.5 (sectored) | 11 | 21 | 4 | 15 |
| Total | 53 | 100 | 27 | 100 |