Table 3. Quantification of second generation of sub-strains obtained from DS10.
Ms from each sub-strain of DS10 (10.1–10.5) was inoculated into DS1, and clones of the induced strains were characterized. DS10.1 largely produced a single predominant strain identical to DS10.1 (92%) and another strain DS10.5 that rapidly sectored (8%). DS10.2–10.4 each recreated all other strains. Columns indicate the number (n) of clones characterized and the percentage of the total (%) in each case. Classification of cell morphology was performed using blinded analysis.
| Induced clone | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 10.1 | 10.2 | 10.3 | 10.4 | 10.5 (sectored) | Total | ||||||||
| Ms | n | % | n | % | n | % | n | % | n | % | n | % | |
| 10.1 | 45 | 92 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 8 | 49 | 100 | |
| 10.2 | 3 | 6 | 37 | 79 | 2 | 4 | 2 | 4 | 3 | 7 | 47 | 100 | |
| 10.3 | 11 | 21 | 3 | 6 | 21 | 42 | 4 | 8 | 12 | 23 | 51 | 100 | |
| 10.4 | 17 | 35 | 3 | 7 | 13 | 27 | 12 | 24 | 3 | 7 | 48 | 100 | |