Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus, C57BL/6, female) |
C57BL/6 | Charles River | Strain code: 027; RRID: IMSR_CRL:27 |
|
| Cell line (M. musculus) |
B16-F10 | doi:10.1038/nm.2753 | RRID: CVCL_0159 |
|
| Cell line (M. musculus) |
B16-F10-luc | doi:10.1038/nm.2753 | expresses firefly luciferase |
|
| Other | synthetic uniloamellar 100 nm liposomes |
Encapsula NanoSciences |
13 mg/ml L-a-Phosplatydilcholine, 2.78 mg/ml cholesterol (7:3 molar ratio P:C) |
|
| Sequence-based reagent |
pGIPZ non-silencing shRNA lentiviral control particles |
Dharmacon/GE Life Sciences | cat# RHS4348 | sense sequence: 5’-ATCTCGCTTGGGCGAGAGTAAG-3’ |
| Sequence-based reagent |
pGIPZ mouse Met shRNA lentiviral particles |
Dharmacon/ GE Life Sciences |
cat# VGM5520- 200377256; clone ID: V3LMM_ 456078 |
sense sequence: 5’-CCAGACTTTTCATACAAGA-3’ |
| Antibody | mouse anti-Hsc70 |
Enzo Life Sciences |
cat# ALX-804–067; RRID: AB_10538284 |
1:500 dilution in SuperBlock, O/N at 4 ˚C |
| Antibody | mouse anti-Tsg101 |
Santa Cruz Biotechnology |
cat# sc-7964; RRID: AB_671392 |
1:500 dilution in SuperBlock, O/N at 4 ˚C |
| Antibody | rabbit anti-Cd63 |
System Biosciences |
cat# EXOAB-CD63A-1; RRID:AB_2561274 |
1:1000 dilution in SuperBlock, O/N at 4 ˚C |
| Antibody | mouse anti-Met | Cell Signaling Technology |
cat# 3127; RRID:AB_331361 |
1:1000 dilution in SuperBlock, O/N at 4 ˚C |
| Antibody | rabbit anti-pMet (Tyr 1234/5) |
Cell Signaling Technology |
cat# 3077; RRID:AB_2315156 |
1:1000 dilution in SuperBlock, O/N at 4 ˚C |
| Antibody | rabbit anti-Gapdh | Santa Cruz Biotechnology |
cat# sc-25778; RRID:AB_10167668 |
1:1000 dilution in SuperBlock, O/N at 4 ˚C |
| Antibody | rabbit anti-pMet (Tyr 1234/5) |
Thermo Fisher Scientific |
cat# MA5-15083; RRID:AB_10983015 |
1:1000 dilution in SuperBlock, O/N at 4 ˚C |
| Antibody | HRP-conjugated sheep anti-mouse |
GE Healthcare | cat# NA931; RRID:AB_772210 |
1:15,000 dilution in SuperBlock, 1 hr at RT |
| Antibody | HRP-conjugated donkey anti-rabbit | GE Healthcare | cat# NA934; RRID:AB_772206 |
1:15,000 dilution in SuperBlock, 1 hr at RT |
| Sequence-based reagent |
shMet primer | this paper | Forward: 5’-CCAGACTTT TCATACAAGAATA-3’ |
|
| Sequence-based reagent |
universal reverse primer |
QuantiMir Kit, System Biosciences |
cat# RA420A-1 | |
| Sequence-based reagent | U6 primer | LncRNA Profiler qPCR Array Kit, System Biosciences |
cat# RA900A-1 | |
| Sequence-based reagent |
Met primer | this paper | Forward: 5’-CTGGACA GATTGTGGGAGTAAG −3’ |
|
| Sequence-based reagent |
Gapdh primer | LncRNA Profiler qPCR Array Kit, System Biosciences | cat# RA900A-1 | |
| Software, algorithm |
ImageJ | doi:10.1038/nmeth.2089 | RRID:SCR_003070 | version 1.49 u |
| Software, algorithm |
SDS | Applied Biosystems |
RRID:SCR_015806 | version 2.4 |
| Software, algorithm |
Nanoparticle tracking Analysis (NTA) |
Nanosight | RRID:SCR_014239 | version 2.3 |
| Software, algorithm |
Living Image | Caliper Life Sciences | RRID:SCR_014247 | version 4.4 |
| Software, algorithm |
R Project for statistical computing |
https://www.r-project.org | RRID:SCR_001905 | version 3.5.1 |