Figure 3. LATS2 directs inner cell mass fate independently of Sox2 (A) Lats2 and GFP or GFP alone were overexpressed in embryos lacking maternal or maternal and zygotic Sox2.

(B) Lats2/GFP-overexpressing cells (dotted line) contribute almost exclusively to the inner cell mass in the presence or absence of Sox2 (n = embryos). (C) Proportion of non-injected cells and cells injected with Lats2/GFP mRNAs contributing to inner cell mass in the indicated genetic backgrounds. No significant differences were observed based on embryo genotype, indicating that Sox2 is dispensable for inside positioning by Lats2-overexpression (P, chi-squared test; n = embryos). (D) Proportion of non-injected cells and cells injected with the indicated mRNAs contributing to trophectoderm in the indicated genetic backgrounds. No significant differences were observed based on embryo genotype (P, chi-squared test; n = embryos).