Figure 15. Impact of SETD3 inactivation on glucose consumption and lactate production in HAP1 cells.

Experiments were performed on three HAP1 cell lines that were deficient in Setd3 (KO-A1, KO-A3 and KO-A5) and a control HAP1 cell line (WT-A6) that has been submitted to the CRISPR/Cas9 gene inactivation procedure, but yielding no modification of the Setd3 gene. HAP1 cells (≈90% culture confluency) were supplemented with fresh medium and incubated for 20 hr as described in the 'Materials and methods'. The glucose and lactate concentrations in samples of deproteinized culture medium were determined spectrophotometrically as described in the 'Materials and methods'. Values are the means ± S.E. (error bars) of three independent experiments performed with cells from three different culture passages (n = 3). The data were assumed to be distributed normally, and the homogeneity of variances was verified with F tests. Statistical significance was analyzed using a one-tailed paired Student’s t-test (GraphPad Prism 4.0). ^#p<0.09, *p<0.05, **p<0.01.