Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | Rabbit anti-SETD3 antibody |
Abcam | ab174662; RRID:AB_2750852 | (1:10,000) |
| Antibody | Rabbit anti-Actin antibody |
Sigma-Aldrich | A2066; RRID:AB_476693 | (1:1000) |
| Antibody | Horseradish peroxidase- conjugated goat anti-rabbit IgG antibody |
Agrisera | AS09602; RRID:AB_1966902 | (1:20,000; 1:25,000) |
| Antibody | Mouse anti-His6 tag antibody |
GE Healthcare; PMID: 23705015 | 27-4710-01; RRID:AB_771435 | (1:2000) |
| Antibody | Horseradish peroxidase -conjugated goat anti-mouse antibody |
Sigma-Aldrich; PMID: 23705015 | A2554; RRID:AB_258008 | (1:10,000) |
| Cell line (Homo sapiens) |
HAP1 | Horizon Discovery | C859; RRID:CVCL_Y019 | The HAP1 cell line was bought directly from Horizon Discovery (Waterbeach, UK) and has been control quality checked by the vendor |
| Cell line (Cercopithecus aethiops) |
COS-7 | Cell Lines Service | 605470; RRID:CVCL_0224 | The COS-7 cell line was bought directly from CLS (Eppelheim, Germany) and has been control quality checked by the vendor |
| Gene (Escherichia coli) |
SAH nucleosidase |
NA | NCBI: NC_000913.3 | |
| Gene (Bacillus subtilis) |
Adenine deaminase |
NA | NCBI: NC_000964.3 | |
| Gene (H. sapiens) |
β-actin | NA | GenBank: NM_001101.4 | |
| Gene (H. sapiens) |
SETD3 | NA | NCBI: NM_032233.2 | |
| Gene (Rattus norvegicus) |
SETD3 | NA | NCBI: XM_002726774.2 | |
| Gene (H. sapiens) |
Cofilin-1 | NA | NCBI: NM_005507.2 | |
| Gene (H. sapiens) |
Profilin-1 | NA | NCBI: NM_005022.3 | |
| Recombinant DNA reagent |
pCOLD I (plasmid) |
Takara Bio | 3361 | |
| Recombinant DNA reagent |
pESC-URA (plasmid) |
Agilent Technologies | 217454 | |
| Recombinant DNA reagent |
pEF6/Myc-His A (plasmid) | Invitrogen | V96220 | |
| Recombinant DNA reagent |
pSpCas0n(BB)−2A-Puro (plasmid) |
Other | Kind gift of F. Zhang, Massachusetts Institute of Technology |
|
| Genetic reagent (C. aethiops) |
pEF6/SETD3 | This paper | Transient overexpression of the recombinant human and rat SETD3 proteins in the COS-7 cell line | |
| Genetic reagent (E. coli) |
pCOLD I/SETD3 | This paper | Overexpression of the recombinant human and rat SETD3 proteins in the E. coli BL21 (DE3) | |
| Genetic reagent (E. coli) |
pCOLD I/β-actin | PMID: 20851184 | Kind gift of M. Tamura, Ehime University | |
| Genetic reagent (E. coli) |
pCOLD I/SAH nucleosidase |
This paper | Overexpression of the recombinant E. coli
SAH nucleosidase in the E.coli BL21 (DE3) |
|
| Genetic reagent (E. coli) |
pCOLD I/adenine deaminase |
This paper | Overexpression of the recombinantB.subtilis adenine deaminase in the E. coli BL21 (DE3) | |
| Genetic reagent (E. coli) |
pCOLD I/Cofilin-1 | This paper | Overexpression of the recombinant human cofilin-1 in the E. coli BL21 (DE3) | |
| Genetic reagent (E. coli) |
pCOLD I/Profilin-1 | This paper | Overexpression of the recombinant human profilin-1 in the E. coli BL21 (DE3) | |
| Genetic reagent (Saccharomyces cerevisiae) |
pESC-URA/β-actin | This paper | Overexpression of the recombinant human β-actin in the S. cerevisiae BY4742 strain | |
| Strain, strain background (B. subtilis) |
Bacillus
subtilis |
Sigma-Aldrich | ATCC:6633 | |
| Strain, strain background (E. coli) |
E. coli BL21 (DE3) | Agilent Technologies | 200131 | |
| Strain, strain background (S. cerevisiae) |
BY4742 | EUROSCARF | Y10000 | |
| Strain, strain background (S. cerevisiae) |
BY4742/β-actin | This paper | BY4742 strain overexpressing recombinant human β-actin |
|
| Peptide, recombinant protein |
Peptide H | Caslo Laboratory | ||
| Peptide, recombinant protein |
Peptide A | Caslo Laboratory | ||
| Peptide, recombinant protein |
Peptide H3N4 | Caslo Laboratory |
||
| Chemical compound, drug |
S-[methyl-3H]adenosyl-L-methionine; [3H]SAM | PerkinElmer | NET155V250UC; NET155V001MC |
|
| Chemical compound, drug |
S-[methyl-2H]adenosyl-L-methionine; [2H]SAM | C/D/N Isotopes Inc. | D-4093 | |
| Software, algorithm |
ProteinLynx Global Server 2.4; PLGS 2.4 |
Waters | RRID:SCR_016664 | |
| Software, algorithm |
Quantity One | BioRad | RRID:SCR_016622 | |
| Software, algorithm |
GraphPad Prism 4.0 |
GraphPad Software | RRID:SCR_002798 | |
| Software, algorithm |
Proteome Discoverer | Thermo Fisher Scientific | RRID:SCR_014477 | |
| Software, algorithm |
Zeiss Zen | Zeiss | RRID:SCR_013672 |