Table 2. Proteins identified in the gel bands submitted to trypsin digestion and MS/MS analysis. Identified proteins are listed for each band according to their score as calculated using ProteinLynx Global Server software (PLGS).
For each protein, the molecular weight (mW) and sequence coverage are also indicated. Occasional peptide hits corresponding to keratins have not been included.
Table 2—source data 1. Mass spectrometry (Q-TOF) identification of proteins present in the gel bands submitted to trypsin digestion.
elife-37921-table2-data1.xls (30.5KB, xls)
DOI: 10.7554/eLife.37921.009
| Gel band | Protein name | NCBI Protein accession number | PLGS score* | mW (Da) |
Coverage (%) |
|---|---|---|---|---|---|
| A | Elongation factor 2 | NP_058941.1 | 16,249 | 95,222 | 73.5 |
| B | Calpain one catalytic subunit | NP_062025.1 | 3024 | 82,067 | 47.7 |
| Elongation factor 2 | NP_058941.1 | 789 | 95,222 | 15.2 | |
| Nuclear protein localization protein four homolog |
NP_542144.1 | 778 | 68,013 | 31.7 | |
| C | Elongation factor 2 | NP_058941.1 | 2024 | 95,222 | 42.1 |
| Nuclear protein localization protein four homolog |
NP_542144.1 | 1263 | 68,013 | 37.3 | |
| Histone lysine N-methyltransferase SETD3 | XP_002726820.2 | 1136 | 67,378 | 39.1 | |
| Eukaryotic peptide chain release factor GTP binding subunit ERF3A |
NP_001003978.1 | 505 | 68,708 | 41.2 | |
| Guanylate binding protein 1 | NP_598308.1 | 404 | 67,312 | 21.8 | |
| Filamin-B-like isoform X2 | XP_003751493.2 | 272 | 121,901 | 1.4 |
*PLGS Score is calculated by the ProteinLynx Global Server (v2.4) software using a Monte Carlo algorithm to analyze all acquired mass spectral data and it is a statistical measure of accuracy of assignation. A higher score implies a greater confidence of protein identity.