Table 4. Analysis of actin methylation at the H74 residue in the tissues of Drosophila larvae by tandem mass spectrometry.
The total number of identified peptide sequences with H74 (peptide spectrum matches, PSMs) for three different forms of actin present in either wildtype Drosophila larvae (WT) or Setd3 KO animals (SETD3 KO) are indicated. The total numbers of PSMs recorded for actin proteins in these MS/MS experiments are as follows: 90 (Actin-42A, WT), 118 (Actin-57B, WT), 111 (Actin-87E, WT), 86 (Actin-42A, SETD3 KO), 111 (Actin-57B, SETD3 KO), 78 (Actin-87E, SETD3 KO).
Table 4—source data 1. Mass spectrometry (Orbitrap Fusion Lumos tribrid) identification of both non-modified and methylated actin peptides from the tissues of Drosophila melanogaster larvae.
elife-37921-fig5.xls (63KB, xls)
DOI: 10.7554/eLife.37921.036