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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Biomaterials. 2018 Oct 25;190-191:24–37. doi: 10.1016/j.biomaterials.2018.10.023

Figure 2.

Figure 2.

Live-cell imaging of human iPSC-derived blood-brain barrier microvessels. (A) representative phase contrast images of a BC1-derived brain microvessel on days two, four and six under a wall shear stress of about 4 dyne cm-2. (B) Phase contrast and fluorescence images of perfusion with Lucifer yellow, Rhodamine 123, and 10 kDa dextran at the microvessel midplane. (C,D) Phase contrast images at the top and bottom planes, respectively. (E) Representative fluorescence intensity for Lucifer yellow for a region of interest comprising both the microvessel and surrounding matrix: (i) Prior to perfusion of the dye. (ii) luminal filling where ΔI represents the increase in fluorescence intensity. (iii) Penetration of the dye into the surrounding matrix results in a linear increase in fluorescence intensity (dI/dt).