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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Biomaterials. 2018 Oct 25;190-191:24–37. doi: 10.1016/j.biomaterials.2018.10.023

Figure 3.

Figure 3.

Permeability of human iPSC-derived blood-brain barrier microvessels. Representative phase / fluorescence overlays of Lucifer yellow (LY), Rhodamine 123 (R123), and 10kDa dextran perfusion in (A) BC1 dhBMEC microvessels, and (B) HUVEC microvessels. t = 0 min represents the frame prior to initiation of luminal filling. Luminal filling occurred on average over 20 minutes for LY and R123, and 30 minutes for 10 kDa dextran. The rate of fluorescence change was determined over subsequent 20 minutes after luminal filling. (C-E) Permeability of LY, R123, and 10 kDa dextran in HUVEC and BC1 dhBMEC microvessels. (FH) Permeability of LY, R123, and 10kDa dextran in dhBMEC microvessels derived from multiple iPS cell lines (BC1, iPS12, KW01, AD6) on day two following seeding. N = 7 BC1 dhBMEC microvessels on day 2, N = 4 BC1 dhBMEC microvessels on day 4 and 6, N = 4 HUVEC microvessels across all timepoints, N = 3 iPSC12, KW01 and AD6 microvessels. *** p < 0.001.