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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2018 Oct 17;362:52–58. doi: 10.1016/j.taap.2018.10.016

Fig. 1. ZIP8 regulates cisplatin response in MEF and HAP1 cells.

Fig. 1.

(A) Total RNA was used for quantitive RT-PCR to determine ZIP8 expression in MEF cells. (B) LUC-MEF and ZIP8-MEF cells were treated with 6, 10 and 20 μg/mL cisplatin for 24 h and phenotype was recorded with an inverted microscope. (C) MEF cell viability was determined by MTT assay after treatment with 10 and 20 μg/mL cisplatin for 24 h. Data is expressed as means ± SE of three independent experiments (N=3). (D) Total RNA was used for quantitative RT-PCR to determine ZIP8 expression in HAP1 cells. (E) HAP1 WT and ZIP8-KO cells were treated with 5, 10 and 20 μg/mL cisplatin for 24 h and phenotype was recorded with an inverted microscope. (F) HAP1 cell viability was determined by MTT assay. Three replicates were used to determine the statistics (N=3). *P<0.05 and **P<0.01.