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. Author manuscript; available in PMC: 2019 Nov 17.
Published in final edited form as: Cytokine. 2018 Nov 17;111:551–562. doi: 10.1016/j.cyto.2018.09.016

Figure 4. EB1089 significantly reduced phosphorylation of STAT1 and STAT3 in NKL cells.

Figure 4.

The NKL cell line was treated with the indicated doses of EB1089, or appropriate negative controls, for 24 h. Total protein loaded per sample was 40 μg with β-actin used as a loading control. Representative western blots for each STAT showing phosphorylated and corresponding total protein are shown: (A) STAT1, (B) STAT2, (C), STAT3, (D) STAT4, (E), STAT5, (F) STAT6. Data from three independent experiments were quantified to determine relative expression levels for phosphorylated STATs 1 – 6 and quantification is shown to the right of each representative western blot. The fold change in the graphs represents the ratio of p-STAT to corresponding total STAT. For STATs with multiple isoforms, the isoform quantifications were combined to give one value for each STAT. Repeated measures ANOVA was used to determine significance of EB1089 treatment compared to 0 nM vehicle control (* p<0.05, ** p<0.01, *** p <0.001). For graphical representation, the vehicle control was set to 1.