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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Biotechnol Bioeng. 2018 Oct 27;116(1):168–180. doi: 10.1002/bit.26839

Figure 4.

Figure 4.

The dose-dependent effects of select combinations on enhanced neuronal specificity relative to astrocyte differentiation by relative percent composition and immunofluorescence-based intensities. Heat maps of the ratio of percentage of TUBB3 positive cells divided by GFAP positive cells in differentiated 3D hNPC cultures during treatment with: (A) RA and CHIR combinations; (B) NPZ and BDNF combinations; and (C) NT3 and NRG1 combinations. Heat maps of the ratio of immunofluorescence-based intensities of spontaneous differentiation normalized neuronal markers (TUBB3+MAP2B) divided by astrocytic markers (GFAP+S100B) of 3D hNPC cultures differentiated with treatment of (D) RA and CHIR combinations, (E) NPZ and BDNF combinations and (F) NT3 and NRG1 combinations.