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Western blot analysis of individual and combined effects of select factors on differentiation outcomes of 100 μL 3D Matrigel ReNcell VM hNPC cultures. (A) Densitometry analysis of western blot detection of (A) TUBB3 and (B) GFAP expression following 3D ReNcell VM differentiation in 100 μL of 1% Matrigel relative to the spontaneous (no factors added) condition. Mean ± SD plotted for n = 2 independent biological replicates.
