. Author manuscript; available in PMC: 2020 Jan 1.

Published in final edited form as: Biotechnol Bioeng. 2018 Oct 27;116(1):168–180. doi: 10.1002/bit.26839

Table 1.

Nierode et al. ANOVA analysis of the individual and combined effects of RA and CHIR on expression of neuronal subtype specific protein markers. Analysis of a dose-response factorial screen of RA, CHIR and combined treatments on expression of pan-neuronal (TUBB3), dopaminergic (TH), GABAergic (GAD65), cholinergic (CHAT) and glutamatergic (VGLUT2) protein markers from two independent screens, wherein each condition had three technical replicates per chip screen (n=6 total replicates per condition and marker). N.S. = non-significant effect. ANOVA from a dose-response.

Media Additive Effects
Protein	RA	CHIR	RA x CHIR
Protein	P-value	P-value	P-value
TUBB3	<0.0001	<0.0001	0.002
TH	<0.05	N.S	N.S
GAD65	<0.001	<0.05	N.S
CHAT	<0.0001	<0.0001	N.S
VGLUT2	<0.0001	<0.001	N.S