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. Author manuscript; available in PMC: 2019 Dec 15.
Published in final edited form as: J Immunol. 2018 Nov 16;201(12):3750–3758. doi: 10.4049/jimmunol.1801041

Figure 1.

Figure 1.

Exosomes derived from ascites fluid of ovarian cancer patients express GD3 on their surface. (A) 2-D TLC was used to identify gangliosides GD3 and GM3 in ascites fluids. Arrows and numbers (right lower corner) indicate the directions of first and second solvent runs. Origin is in the lower right. Ganglioside standards, noted along the top (solvent 1) and left (solvent 2) margins, are: GM1 (II3NeuAc-GgOse4Cer); GD1a (IV3NeuAc,II3NeuAc-GgOse4Cer); GD1b (II3(NeuAc)2-GgOse4Cer; GT1b (IV3NeuAc-II3(NeuAc)2-GgOse4Cer). Standards on the right margin include GM3 (II3NeuAc-LacCer); GM2 (II3NeuAc-GgOse3Cer); GM1; GD3 (II3(NeuAc)2-LacCer); GD1a; GD1b. (B) Exosomes were attached to latex beads and either left unstained (filled histogram), labeled with secondary antibody only (dotted line) or with anti-GD3 antibody (solid line) and data acquired using a flow cytometer. Data are representative of 3 independent experiments.