Figure 2.

Immunodepletion of GD3+ exosomes diminishes exosome-mediated T cell arrest. (A) NDPBL were either left unactivated (Unact) or activated for 2h with immobilized antibodies to CD3 and CD28 in media only (Act) or in media with total exosomes (Exo) or exosomes subjected to depletion using either anti-GD3 antibody coupled magnetic beads (GD3 Dep) or isotype control coupled magnetic beads (Iso Dep). Activation was determined by monitoring the upregulation of CD69 on live T cells by flow cytometry following overnight incubation. Representative experiment shown. (B) Compiled data from 5 experiments is shown. Numbers in parentheses indicate % inhibition. (C) Calculated % knockdown of inhibition is shown. Mean ± SEM. NS = Not significant (p > 0.05). * p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.