Figure 6.

Sialidase treatment of GD3 liposomes knocks down T cell arrest. NDPBL were either left unactivated or activated for 2h with immobilized antibodies to CD3 and CD28 in media only or in the presence of 1 mM PC or GD3/PC on liposomes that were untreated or treated with 0.8 U/mL sialidase. A control group was also included where the cells were activated in the presence of the same concentration of sialidase without any liposomes present. Activation was determined by counting the number of CD3+ cells with nuclear NFκB using confocal microscopy. Compiled data from 3 experiments is shown. There was a 97% knockdown of the T cell arrest. Mean ± SEM. n = 3. NS = Not significant (p > 0.05), ***p ≤ 0.001.