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. Author manuscript; available in PMC: 2019 Jun 3.
Published in final edited form as: Nat Chem Biol. 2018 Dec 3;15(1):11–17. doi: 10.1038/s41589-018-0160-y

Figure 2|. Detailed structure of the misoprostol-FA binding pocket and interactions of the ligand with EP3 receptor.

Figure 2|

(a) Two-dimensional view of receptor interactions with misoprostol-FA. Polar and hydrophobic interactions are shown in blue and green boxes, respectively. (b) Detailed view of misoprostol-FA interactions. Polar interactions are shown as dotted lines. Misoprostol-FA is shown as green sticks. Water and Gly1413.36 are shown as red and gold spheres, respectively. Side chains coordinating the α-chain, ω-chain, and E-ring regions are shown as blue, gold, and maroon carbons, respectively. (c,d) Misoprostol-FA Gi signaling on wild-type (WT) and EP3 receptor mutants. Values are mean ± SEM of 6 and 3 independent experiments for the WT and mutants, respectively. Significance of the basal value was determined using one-way ANOVA analysis followed by a Dunnett post-hoc test against the mean basal WT signaling. For the ANOVA, p < 0.0001, F is 62.07, among and within group degrees of freedom are 3 and 11, respectively. Post-hoc test p values are indicated on the graph. Mean pEC50 ± SEM are shown in Supplementary Table 3.