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. Author manuscript; available in PMC: 2020 Feb 1.
Published in final edited form as: Magn Reson Med. 2018 Sep 8;81(2):1280–1295. doi: 10.1002/mrm.27473

Figure 5.

Figure 5.

Bath-application of extracellular ionotropic glutamate receptor antagonists blocks spontaneous neuronal activity and decreases AWC. (A) Spontaneous neuronal excitation (basal) is blocked by 10 μM DNQXo plus 50 μM AP5,o, and recovers after washout. Time-courses of representative continuous population calcium fluorescence signals in response to AP5 plus DNQX and washout with normal ACSF. (B) Significant decreases in mean fluorescent activity <∆F/F0>n, (left), mean cellular water efflux rate constant 〈kio〉n (middle), and intracellular water mole fraction 〈pi〉n (right) when spontaneous activity is blocked. Basal (n = 10), AP5 plus DNQX perfusion (n = 10), and washout with normal ACSF (n = 7). In the boxplots, box edges represent 25th and 75th percentiles, the middle line is the median, and whisker length is 1.5 times the box length. N. S. represents non-significant with p ≥ 0.05.