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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: FEBS J. 2018 Oct 25;285(23):4378–4393. doi: 10.1111/febs.14677

Figure 6. The AID system enables degradation of proteins in nervous system of adult Drosophila.

Figure 6.

(A) Representative LNd, DN1, and LNvs stained with GFP and PER at CT1 to confirm degradation of PER in AID-TG4-TIR flies after 0 mM and 25 mM auxin treatments. (B) GFP intensities from analyses of AID-TG4-TIR flies after 0 mM (n=8) and 25 mM (n=10) auxin treatments. Flies were fed with auxin for 3 days and then transferred to constant dark (DD) condition. Samples were collected for staining on the first day of DD at CT1. (C) Representative LNd, DNs, and LNvs stained with GFP and VRI at ZT18 after the third day with or without auxin treatment to confirm the normality of circadian neurons (n=8).