Fig. 5.
Competition binding experiments. (a) The indicated concentrations of unlabeled competitor 28-bp oligos were incubated with 0.25 μM of TRIM25 in complex with fluorescent 28-bp RNA and electrophoretic migration of the fluorescent probe was analyzed. (b) The amounts of fluorescent probe displaced by 5 μM competitor were quantified and plotted. Experiments were repeated three times independently, and representative results are shown.