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. 2018 Sep 4;25(6):597–617. doi: 10.1093/dnares/dsy028

Figure 5.

Figure 5.

Quantitative RT-PCR validation of expression of terpene synthase genes selected from the DGE analysis in S. guaranitica. Total RNAs were extracted from old leaves, young leaves, stem, flower, bud flower and root samples and the expression of SgGPPS, SgFPPS, SgHUMS, SgNEOD-1, SgNEOD-2, SgNEOD-3, SgTPS-1, SgTPS-3, SgTPS-6, SgLINS-1, SgLINS-2, SgGLNS, SgGERIS, SgTPS-V and SgFARD genes were analysed using quantitative real-time. SgACTIN was used as the internal reference. The values are means ± SE of three biological replicates.